CATALOG NUMBER: C1336

QUANTITY: 50µL

DESCRIPTION:

S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). When RNase A is digested with subtilisin, a single peptide bond is cleaved. The resulting two products remain weakly bound to each other and the protein called ribonuclease S remains active. However each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag of which the amino acid sequence is: KETAAAKFERQHMDS.

SPECIFICITY: The S tag antibody can recognize S tag fusion proteins.

ISOTYPE: IgG1

TESTED APPLICATIONS: Western Blot

APPLICATIONS: WB: 1:5,000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Western Blot

2ug S-Tag fusion protein+ Primary antibody dilution at     1、1:5,000      2、1:10,000

For research use only; not for use as a diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently dislodge any liquid in the container^,‘s cap.

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