CATALOG NUMBER: C1817

QUANTITY: 50µL

DESCRIPTION:

E-Cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis.

SPECIFICITY: Rabbit

SPECIES REACTIVITY: human, mouse, rat

ANTIGEN: a 17 amino acid synthetic peptide from the middle of the human E-cadherin

FORMAT: affinity purified using corresponding antigen

CONCENTRATION: 0.2 mg/mL

ISOTYPE: IgG

MOLECULAR WEIGHT: ~120KDa

TESTED APPLICATIONS: WB, IF, IP

APPLICATIONS: WB: 1:4,000-1:8,000            IF: 1:100-1:400

IP: 5ug for 500 ug of cell lysate.

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Product images:

A: Western blot analysis: line 1: 40 ug cell lysate from mouse small intestine epithelia; line 2: 40 ug cell lysate from human HT29 cell line.

B: Confocal images of stained human bronchial epithelial cells. XY section shows that E-cadherin is localized on the plasma membrane; XZ section shows that it is localized on the lateral membrane.

For research use only; not for use as a diagnostic.

Important Note:

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently dislodge any liquid in the container^,‘s cap.