CATALOG NUMBER: C1833

QUANTITY: 50µL

DESCRIPTION:

The Akt proteins (Akt1, Akt2 and Akt3) are a family of cytoplasmic serine-threonine kinases that form a critical junction at the intersection of multiple cell signaling pathways. Also known as PKB, Akt is activated by multiple extracellular signals by phosphorylation at specific residues and is subsequently translocated to the plasma membrane. Akt is involved in the PI3K/AKT/mTOR pathway and other signaling pathways. The oncogenic properties of the Akt proteins are seen following mutation or constitutive activation in several different malignancies. Activity of the Akt proteins is negatively by PTEN, a protein tyrosine phosphatase tumor suppressor. Akt has also been shown to function as a survival factor in many cell types, an activity mediated by the phosphorylation of members of the bcl-2 family of proteins such as BAD.

SPECIES REACTIVITY: Human, Mouse, Rat.

MOLECULAR WEIGHT: ~60 kDa

ISOTYPE: IgG

TESTED APPLICATIONS: Western Blot

APPLICATIONS: WB: 1:5,000-1:10,000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

For research use only; not for use as a diagnostic.

Important Note:

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently dislodge any liquid in the container^,‘s cap.