CATALOG NUMBER: C2225

SIZE: 50µg/100µL

DESCRIPTION: Affinity purified antibody isolated from a pool of serum from goats immunized with purified mouse IgG was labeled with peroxidase by the periodate method of Nakane and Kawaoi^[1]

FORM/STORAGE: Store at -20°C for a minimum of 1 year.

STABILIZER AND PRESERVATIVE: Goat serum added as a protein stabilizer. No preservative added. Additional biological protection may be provided with 0.01% thimerosal. DO NOT USE SODIUM AZIDE. Non-sterile.

ANTIBODY CONCENTRATION: The concentration of affinity purified antibody is 1.0mg as determined by UV absorbance at 280 nm.

E/P RATIO: Molar enzyme/antibody protein ratio = 4:1

SPECIFICITY/CROSS REACTIVITY: 

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.   

SUGGESTED WORKING DILUTIONS: 

Optimal working concentrations should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution (See RELATED PRODUCTS). These buffers not recommended for long term storage.  Suggested starting dilutions are as follows.

In many cases, the antibody may be diluted further than indicated.

ELISA:    1:2500 - 1:50,000                

Blotting:   1:5000 - 1:100,000          

Histo/Cytochemical Procedures:  1:250-1:2,500

*NOTE: Optimal working dilutions must be determined by the end user

PRODUCT SAFETY AND HANDLING: This product is considered non-hazardous as defined by the Hazard Communication Standard (29 CFR 1910.1200). Avoid contact with skin and eyes. In case of contact or spillage, clean with copious amounts of water. Disposal via sanitary sewer. 

REFERENCES

1.  Nakane, P K., and Kawaoi, A. (1974). J. Histochem. A.1974). J. Histochem. Cytochem. 22:1084. 

For research use only; not for use as a diagnostic.

Important Note: 

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.