CATALOG NUMBER: C1321

QUANTITY: 50µL

DESCRIPTION:

C-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. Myc protein is a transcription factor that activates expression of a great number of genes through binding on consensus sequences (Enhancer Box sequences (E-boxes)) and recruiting histone acetyltransferases (HATs). A recent study demonstrate that temporary inhibition of Myc selectively kills mouse lung cancer cells, making it a potential cancer drug target.

SPECIFICITY: The C-Myc tag antibody can detect C-Myc fusion proteins.

ISOTYPE: IgG1

TESTED APPLICATIONS: Western Blot,  Immunoprecipitation, Immunofluorescense

APPLICATIONS: WB: 1:5,000    IP: 1:200    IF: 1:1000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Immunoprecipitation:

IP antibody use：3ug C-Myc Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate          2、transfected 293 cell lysate with C-Myc-tag fusion protein

3、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

4、IP (transfected 293+anti- C-Myc mAb+ Protein G agarose)

5、IP (transfected 293+Protein G)

6、Recombinant protein (E.coli)

Western Blot Immunofluorescense

1ug C-Myc fusion protein+ Primary antibody dilution at IF analysis of 293 cells transfected with

1、1:5,000                                         a C-Myc-tag protein, using anti-

2、1:10,000 C-Myc Tag Mouse mAb at a 1:2000

dilution (blue DAPI, red anti-C-Myc)

For research use only; not for use as a diagnostic.

Important Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.

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