CATALOG NUMBER: C1304

QUANTITY: 100µL

DESCRIPTION:

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.

SPECIFICITY: The HA tag antibody can recognize C-terminal, internal, and N-terminal HA-tag fusion proteins.

ISOTYPE: IgG1

TESTED APPLICATIONS: Western Blot,  Immunoprecipitation, Immunofluorescense

APPLICATIONS: WB: 1:1,000- 1:8,000   IP: 1:200    IF: 1:1000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Immunoprecipitation:

IP antibody use：5ug HA Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate          2、transfected 293 cell lysate with HA-tag fusion protein

3、IP (untransfected 293+anti- HA mAb+ Protein G agarose)

4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5、IP (transfected 293+anti-HA mAb+ Protein G agarose)

6、IP (transfected 293+Protein G)        7、Recombinant protein (E.coli)

Western Blot Immunofluorescense

0.5ug HA fusion protein+ Primary antibody dilution IF analysis of 293 cells transfected with

at 1:10,000              a HA-tag protein, using anti-

HA-Tag Mouse mAb at a 1:2000

dilution (blue DAPI, red anti-HA)

For research use only; not for use as a diagnostic.

Important Note:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.

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