CATALOG NUMBER: C1301

QUANTITY: 100µl   250µl

DESCRIPTION:

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

SPECIFICITY:

The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.

ISOTYPE: IgG1

TESTED APPLICATIONS: Western Blot  Immunoprecipitation Immunofluorescense

IP   ELISA

APPLICATIONS: Western blot: 1:1,000-1:8,000

Immunoprecipitation or immunostaining: 1:500-1：2,000

ELISA on recombinant proteins: 1:500-1:2,000

*NOTE: Optimal working dilutions must be determined by the end user

STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Immunoprecipitation:

IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000

1、untransfected 293 cell lysate          2、transfected 293 cell lysate with His-tag fusion protein

3、IP (untransfected 293+anti-His mAb+ Protein G agarose)

4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

5、IP (transfected 293+anti-His mAb+ Protein G agarose)

6、Recombinant protein (E.coli)

Western Blot Immunofluorescense

1ug His fusion protein+ Primary antibody dilution IF analysis of 293 cells transfected with

at                                                    a His tag protein, using anti-

1、1:5,000                                        His-Tag Mouse mAb at a 1:2000

2、1:10, 000 dilution (blue DAPI, red anti-His)

For research use only; not for use as a diagnostic.

Important Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.

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