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磷酸酶抑制剂混合物通用型(All-in-One,100x) P1260厂家直销，大包装更优惠！
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- 引用文献
描述:
组织细胞在裂解过程中释放大量的内源性蛋白磷酸酶，能够以各种方式催化磷酸化蛋白的去磷酸化。本蛋白磷酸酶抑制剂混合物(All-in-One, 100x)包含6种独立的蛋白磷酸酶抑制剂，每一种抑制剂可特异性抑制某一种或几种蛋白磷酸酶活性，包括蛋白丝/苏氨酸磷酸酶(PP1、PP2A、PP2B、PP2C)、蛋白酪氨酸磷酸酶(PTPs)、碱性磷酸酶、酸性磷酸酶等。该混合物为100倍浓缩水溶液，可直接加入任何类型组织和细胞的裂解产物中，有效抑制磷酸化蛋白质的去磷酸化，从而维护蛋白质的磷酸化状态。适用于Western Blot和免疫共沉淀检测磷酸化蛋白质、测定蛋白激酶活性等实验。
适用：
Western Blot和免疫共沉淀检测磷酸化蛋白质、蛋白激酶活性测定等实验。
组成:
混合物为100倍浓缩水溶液，包含6种独立的蛋白磷酸酶抑制剂；强烈抑制所有蛋白磷酸酶的活性，包括蛋白丝/苏氨酸磷酸酶(PP1、PP2A、PP2B、PP2C)、蛋白酪氨酸磷酸酶(PTPs)、碱性磷酸酶、酸性磷酸酶等。
储存:
4℃12个月有效。储存期间溶液如果出现灰色，不影响使用。
规格：
1ml 5ml
使用方法：
1. 取出蛋白磷酸酶抑制剂混合物(All-in-One,100x)，振荡重悬。
2. 按照1:99比例，加入组织或细胞裂解液中，混合均匀 。
以下列举部分使用普利莱蛋白磷酸酶抑制剂发表的SCI文章供参考：
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