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膜再生液(抗体去除液、抗体剥离液) 温和型 P1650厂家直销，提供OEM定制服务！
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描述：
膜再生液，也称做抗体剥离液体、一抗二抗去除液。允许对同一张膜进行多次Western Blot检测。在室温条件下，将用过的膜浸泡在膜再生液中30分钟，能够选择性清除与膜抗原结合的第一抗体及第二抗体，但不影响电转移到膜上的蛋白。随后可以使用不同的抗体进行下一轮Western Blot实验，可利用同一张膜进行多次蛋白检测。不仅适宜用少量样品多次检测多种不同蛋白，即使样品量并不匮乏，采用这种多次检测同一张膜上的蛋白的策略，能省去给药处理、蛋白电泳和膜转移等耗费性步骤。
特点：
（1）无毒无味无害，室温保存和使用；
（2）可对同一张膜进行10次以上的Western Blot检测。
适用：
硝酸纤维素膜或PVDF膜。使用前膜可以保存在PBS或TBS缓冲液，也可以室温干燥保存数月。
干的PVDF膜应该先用甲醇泡5分钟。
安全性：
无毒无味。按照普通化学品安全规范进行操作和处置。
储存：
室温保存，一年有效。温度过低可能出现浑浊。
效果展示：
第一次Western Blot 第一次Western Blot后用膜再生液处理，然后进行第二次Western Blot
操作步骤：
1. 将膜充分浸泡于适当体积的再生液中，室温孵育15~30分钟并不时晃动。孵育时间的长短应参考后面的说明进行优化。洗脱某些抗体需要较长的时间如30~60分钟。
2. 用镊子取出膜，用自备Western Blot洗涤缓冲液或普利莱封闭洗涤缓冲液（B1009）淋洗膜一次，再洗膜5分钟。
3. 此时膜上抗体已去除，膜已再生。用脱脂奶粉或BSA封闭，进行下一轮Western Blot实验。
说明:
1. 膜再生或Stripping的实质是在不影响膜上结合的抗原的条件下，将与抗原分子结合的一抗和二抗洗脱下来。有许多因素影响抗体从膜上的洗脱，如膜的类型、抗体类型和浓度及其与抗原结合特性等。按下面的说明优化再生液中孵育膜的时间至关重要。
2. 确定膜上抗体是否去除与优化再生液中孵育膜的时间：用ECL工作液孵育再生后的膜约1分钟，然后进行X光胶片曝光并显影，可确定膜上的抗体是否完全去除。如显示条带，表明抗体未完全去除，应继续将膜浸泡在再生液中孵育30~60分钟。然后再次ECL 检查膜上抗体是否去除。重复此步骤直到抗体完全去除并确定最佳孵育时间。
3. 由于至今尚不清楚的原因，使用脱脂奶粉封闭的膜要比使用BSA封闭的膜上的抗体更容易被Strip下来。因此准备进行Strip的膜，应该使用脱脂奶粉而不是BSA封闭。
4. 尽量避免使用干燥保存的膜，因为干的膜上的抗体很难被Strip干净。
部分使用普利莱膜再生液发表的SCI文章，供参考：
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18. Liu T, Li T, Chen X, et al. EETs/sEHi alleviates nociception by blocking the crosslink between endoplasmic reticulum stress and neuroinflammation in a central poststroke pain model[J]. Journal of Neuroinflammation, 2021, 18: 1-19.
19. Dang Y, An Y, He J, et al. Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression[J]. Aging Cell, 2020, 19(1): e13060.
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22. Dang Y, An Y, He J, et al. Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression[J]. Aging Cell, 2020, 19(1): e13060.
23. Zu L, Jiang H, He J, et al. Salicylate blocks lipolytic actions of tumor necrosis factor-α in primary rat adipocytes[J]. Molecular pharmacology, 2008, 73(1): 215-223.
24. Liu T, Li T, Chen X, et al. EETs/sEHi alleviates nociception by blocking the crosslink between endoplasmic reticulum stress and neuroinflammation in a central poststroke pain model[J]. Journal of Neuroinflammation, 2021, 18: 1-19.
25. Zhai W, Xu C, Ling Y, et al. Increased lipolysis in adipose tissues is associated with elevation of systemic free fatty acids and insulin resistance in perilipin null mice[J]. Hormone and metabolic research, 2010, 42(04): 247-253.
26. Ren T, He J, Jiang H, et al. Metformin reduces lipolysis in primary rat adipocytes stimulated by tumor necrosis factor-a or isoproterenol[J]. Journal of molecular endocrinology, 2006, 37(1): 175-184.
27. Zhao X, Gao M, He J, et al. Perilipin1 deficiency in whole body or bone marrow-derived cells attenuates lesions in atherosclerosis-prone mice[J]. PLoS One, 2015, 10(4): e0123738.
28. Deng J, Liu S, Zou L, et al. Lipolysis response to endoplasmic reticulum stress in adipose cells[J]. Journal of Biological Chemistry, 2012, 287(9): 6240-6249.
29. Zhang T, He J, Xu C, et al. Mechanisms of metformin inhibiting lipolytic response to isoproterenol in primary rat adipocytes[J]. Journal of molecular endocrinology, 2009, 42(1): 57-66.
30. Liu T, Li T, Chen X, et al. A network-based analysis and experimental validation of traditional Chinese medicine Yuanhu Zhitong Formula in treating neuropathic pain[J]. Journal of Ethnopharmacology, 2021, 274: 114037.
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